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mouse anti human pmlc2 ser19  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc mouse anti human pmlc2 ser19
    a – i Physical interventions altering lumen and branch expansions by externally loading intraluminal pressure in on-chip angiogenesis of ECs. a A schematic diagram showing the timeline of the loading and release cycles of intraluminal pressure. b , c Changes of lumens and branches when loaded followed by release of the additional intraluminal pressure. b Representative DIC images. White dotted lines indicate the proximal and distal boundaries of the analyzed luminal region. c Quantification of the changes in luminal area (loading: n = 12; release: n = 11, from 3 independent experiments). d – i Time-lapse analyses of on-chip angiogenesis during the intervention. d Representative serial DIC images at the elapsed time indicated at the top before and after loading and the release of additional intraluminal pressure (top, See also Supplementary Movie ) and kymograph of branch elongation (bottom). In the DIC images, yellow arrows and red lines indicate the trajectory of the tip of the branch and the tip EC displacement, respectively. In the kymograph, red and blue dotted lines indicate time points at which additional intraluminal pressure was loaded and then released, respectively. e Box plots of averaged branch elongation and the individual plots 3 h before (pressure change (−)) and after (load) the 1 st pressure loading and 3 h after its release (release) (left), and the corresponding box and individual plots in the control group without interventions (right) (pressure loading and release: n = 21 from 4 independent experiments, control: n = 19, from 3 independent experiments, See also Supplementary Fig. ). f – i Kymograph showing the dynamics of tip EC movement for 50 min before and 100 min after loading ( f ) and releasing ( h ) additional intraluminal pressure (time=0, dotted red line), and the quantification showing in box plots of averaged tip EC movement speed ( g , each group: n = 13 from 4 independent experiments; i , each group: n = 8 from 4 independent experiments). j – m Pharmacological interventions altering lumen and branch expansions of on-chip angiogenesis of ECs by treatment with Y27632 (top) and Blebbistatin (bottom), and without and with TG treatment of the gel. j Representative fluorescent images of the angiogenic branches. k Representative confocal z-projection images, with magnified images of square areas bound by dots (z-projection) and their corresponding confocal x-y slice images at specific z-portions (slice), showing localization pattern of <t>pMLC2</t> in the angiogenic branches around the area where the de novo vascular lumen (yellow asterisks) develops. l , m , Box plots of branch length (left) and Tip expandability (right), without and with Y27632 ( l ) or Blebbistatin ( m ) and without and with TG treatment of the gel ( l , Y27632 (−) TG (−): n = 117; Y27632 ( + ) TG (−): n = 108; Y27632 ( + ) TG (+): n = 102, from 3 independent experiments, ( m ), Blebbistatin (−) TG (−): n = 39; Blebbistatin (+) TG (−): n = 35; Blebbistatin (+) TG (+): n = 64, from 3 independent experiments). All box plots show the interquartile range, with the middle line defining the median, and whiskers show the minimum and maximum values, excluding outliers ( g , i , l , m ). Outlier values are defined as being 1.5 times the interquartile range above and below the third and first quartile, respectively. Scale bars: 20 µm ( d , k ), 25 µm ( b ) and 100 µm ( j ). Two-sided Wilcoxon signed rank test ( c ), Wilcoxon signed rank test with a Bonferroni correction ( e ) and two-sided Mann-Whitney U test with a Bonferroni correction ( g , i , l , m ). Source data are provided as a Source Data file.
    Mouse Anti Human Pmlc2 Ser19, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+pmlc2+ser19/pmc12304211-309-65-69?v=Cell+Signaling+Technology+Inc
    Average 86 stars, based on 1 article reviews
    mouse anti human pmlc2 ser19 - by Bioz Stars, 2026-08
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    Images

    1) Product Images from "Biomechanical control of vascular morphogenesis by the surrounding stiffness"

    Article Title: Biomechanical control of vascular morphogenesis by the surrounding stiffness

    Journal: Nature Communications

    doi: 10.1038/s41467-025-61804-z

    a – i Physical interventions altering lumen and branch expansions by externally loading intraluminal pressure in on-chip angiogenesis of ECs. a A schematic diagram showing the timeline of the loading and release cycles of intraluminal pressure. b , c Changes of lumens and branches when loaded followed by release of the additional intraluminal pressure. b Representative DIC images. White dotted lines indicate the proximal and distal boundaries of the analyzed luminal region. c Quantification of the changes in luminal area (loading: n = 12; release: n = 11, from 3 independent experiments). d – i Time-lapse analyses of on-chip angiogenesis during the intervention. d Representative serial DIC images at the elapsed time indicated at the top before and after loading and the release of additional intraluminal pressure (top, See also Supplementary Movie ) and kymograph of branch elongation (bottom). In the DIC images, yellow arrows and red lines indicate the trajectory of the tip of the branch and the tip EC displacement, respectively. In the kymograph, red and blue dotted lines indicate time points at which additional intraluminal pressure was loaded and then released, respectively. e Box plots of averaged branch elongation and the individual plots 3 h before (pressure change (−)) and after (load) the 1 st pressure loading and 3 h after its release (release) (left), and the corresponding box and individual plots in the control group without interventions (right) (pressure loading and release: n = 21 from 4 independent experiments, control: n = 19, from 3 independent experiments, See also Supplementary Fig. ). f – i Kymograph showing the dynamics of tip EC movement for 50 min before and 100 min after loading ( f ) and releasing ( h ) additional intraluminal pressure (time=0, dotted red line), and the quantification showing in box plots of averaged tip EC movement speed ( g , each group: n = 13 from 4 independent experiments; i , each group: n = 8 from 4 independent experiments). j – m Pharmacological interventions altering lumen and branch expansions of on-chip angiogenesis of ECs by treatment with Y27632 (top) and Blebbistatin (bottom), and without and with TG treatment of the gel. j Representative fluorescent images of the angiogenic branches. k Representative confocal z-projection images, with magnified images of square areas bound by dots (z-projection) and their corresponding confocal x-y slice images at specific z-portions (slice), showing localization pattern of pMLC2 in the angiogenic branches around the area where the de novo vascular lumen (yellow asterisks) develops. l , m , Box plots of branch length (left) and Tip expandability (right), without and with Y27632 ( l ) or Blebbistatin ( m ) and without and with TG treatment of the gel ( l , Y27632 (−) TG (−): n = 117; Y27632 ( + ) TG (−): n = 108; Y27632 ( + ) TG (+): n = 102, from 3 independent experiments, ( m ), Blebbistatin (−) TG (−): n = 39; Blebbistatin (+) TG (−): n = 35; Blebbistatin (+) TG (+): n = 64, from 3 independent experiments). All box plots show the interquartile range, with the middle line defining the median, and whiskers show the minimum and maximum values, excluding outliers ( g , i , l , m ). Outlier values are defined as being 1.5 times the interquartile range above and below the third and first quartile, respectively. Scale bars: 20 µm ( d , k ), 25 µm ( b ) and 100 µm ( j ). Two-sided Wilcoxon signed rank test ( c ), Wilcoxon signed rank test with a Bonferroni correction ( e ) and two-sided Mann-Whitney U test with a Bonferroni correction ( g , i , l , m ). Source data are provided as a Source Data file.
    Figure Legend Snippet: a – i Physical interventions altering lumen and branch expansions by externally loading intraluminal pressure in on-chip angiogenesis of ECs. a A schematic diagram showing the timeline of the loading and release cycles of intraluminal pressure. b , c Changes of lumens and branches when loaded followed by release of the additional intraluminal pressure. b Representative DIC images. White dotted lines indicate the proximal and distal boundaries of the analyzed luminal region. c Quantification of the changes in luminal area (loading: n = 12; release: n = 11, from 3 independent experiments). d – i Time-lapse analyses of on-chip angiogenesis during the intervention. d Representative serial DIC images at the elapsed time indicated at the top before and after loading and the release of additional intraluminal pressure (top, See also Supplementary Movie ) and kymograph of branch elongation (bottom). In the DIC images, yellow arrows and red lines indicate the trajectory of the tip of the branch and the tip EC displacement, respectively. In the kymograph, red and blue dotted lines indicate time points at which additional intraluminal pressure was loaded and then released, respectively. e Box plots of averaged branch elongation and the individual plots 3 h before (pressure change (−)) and after (load) the 1 st pressure loading and 3 h after its release (release) (left), and the corresponding box and individual plots in the control group without interventions (right) (pressure loading and release: n = 21 from 4 independent experiments, control: n = 19, from 3 independent experiments, See also Supplementary Fig. ). f – i Kymograph showing the dynamics of tip EC movement for 50 min before and 100 min after loading ( f ) and releasing ( h ) additional intraluminal pressure (time=0, dotted red line), and the quantification showing in box plots of averaged tip EC movement speed ( g , each group: n = 13 from 4 independent experiments; i , each group: n = 8 from 4 independent experiments). j – m Pharmacological interventions altering lumen and branch expansions of on-chip angiogenesis of ECs by treatment with Y27632 (top) and Blebbistatin (bottom), and without and with TG treatment of the gel. j Representative fluorescent images of the angiogenic branches. k Representative confocal z-projection images, with magnified images of square areas bound by dots (z-projection) and their corresponding confocal x-y slice images at specific z-portions (slice), showing localization pattern of pMLC2 in the angiogenic branches around the area where the de novo vascular lumen (yellow asterisks) develops. l , m , Box plots of branch length (left) and Tip expandability (right), without and with Y27632 ( l ) or Blebbistatin ( m ) and without and with TG treatment of the gel ( l , Y27632 (−) TG (−): n = 117; Y27632 ( + ) TG (−): n = 108; Y27632 ( + ) TG (+): n = 102, from 3 independent experiments, ( m ), Blebbistatin (−) TG (−): n = 39; Blebbistatin (+) TG (−): n = 35; Blebbistatin (+) TG (+): n = 64, from 3 independent experiments). All box plots show the interquartile range, with the middle line defining the median, and whiskers show the minimum and maximum values, excluding outliers ( g , i , l , m ). Outlier values are defined as being 1.5 times the interquartile range above and below the third and first quartile, respectively. Scale bars: 20 µm ( d , k ), 25 µm ( b ) and 100 µm ( j ). Two-sided Wilcoxon signed rank test ( c ), Wilcoxon signed rank test with a Bonferroni correction ( e ) and two-sided Mann-Whitney U test with a Bonferroni correction ( g , i , l , m ). Source data are provided as a Source Data file.

    Techniques Used: Control, MANN-WHITNEY



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    mouse anti human pmlc2 ser19  (Cell Signaling Technology Inc)
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    Cell Signaling Technology Inc mouse anti human pmlc2 ser19
    a – i Physical interventions altering lumen and branch expansions by externally loading intraluminal pressure in on-chip angiogenesis of ECs. a A schematic diagram showing the timeline of the loading and release cycles of intraluminal pressure. b , c Changes of lumens and branches when loaded followed by release of the additional intraluminal pressure. b Representative DIC images. White dotted lines indicate the proximal and distal boundaries of the analyzed luminal region. c Quantification of the changes in luminal area (loading: n = 12; release: n = 11, from 3 independent experiments). d – i Time-lapse analyses of on-chip angiogenesis during the intervention. d Representative serial DIC images at the elapsed time indicated at the top before and after loading and the release of additional intraluminal pressure (top, See also Supplementary Movie ) and kymograph of branch elongation (bottom). In the DIC images, yellow arrows and red lines indicate the trajectory of the tip of the branch and the tip EC displacement, respectively. In the kymograph, red and blue dotted lines indicate time points at which additional intraluminal pressure was loaded and then released, respectively. e Box plots of averaged branch elongation and the individual plots 3 h before (pressure change (−)) and after (load) the 1 st pressure loading and 3 h after its release (release) (left), and the corresponding box and individual plots in the control group without interventions (right) (pressure loading and release: n = 21 from 4 independent experiments, control: n = 19, from 3 independent experiments, See also Supplementary Fig. ). f – i Kymograph showing the dynamics of tip EC movement for 50 min before and 100 min after loading ( f ) and releasing ( h ) additional intraluminal pressure (time=0, dotted red line), and the quantification showing in box plots of averaged tip EC movement speed ( g , each group: n = 13 from 4 independent experiments; i , each group: n = 8 from 4 independent experiments). j – m Pharmacological interventions altering lumen and branch expansions of on-chip angiogenesis of ECs by treatment with Y27632 (top) and Blebbistatin (bottom), and without and with TG treatment of the gel. j Representative fluorescent images of the angiogenic branches. k Representative confocal z-projection images, with magnified images of square areas bound by dots (z-projection) and their corresponding confocal x-y slice images at specific z-portions (slice), showing localization pattern of <t>pMLC2</t> in the angiogenic branches around the area where the de novo vascular lumen (yellow asterisks) develops. l , m , Box plots of branch length (left) and Tip expandability (right), without and with Y27632 ( l ) or Blebbistatin ( m ) and without and with TG treatment of the gel ( l , Y27632 (−) TG (−): n = 117; Y27632 ( + ) TG (−): n = 108; Y27632 ( + ) TG (+): n = 102, from 3 independent experiments, ( m ), Blebbistatin (−) TG (−): n = 39; Blebbistatin (+) TG (−): n = 35; Blebbistatin (+) TG (+): n = 64, from 3 independent experiments). All box plots show the interquartile range, with the middle line defining the median, and whiskers show the minimum and maximum values, excluding outliers ( g , i , l , m ). Outlier values are defined as being 1.5 times the interquartile range above and below the third and first quartile, respectively. Scale bars: 20 µm ( d , k ), 25 µm ( b ) and 100 µm ( j ). Two-sided Wilcoxon signed rank test ( c ), Wilcoxon signed rank test with a Bonferroni correction ( e ) and two-sided Mann-Whitney U test with a Bonferroni correction ( g , i , l , m ). Source data are provided as a Source Data file.
    Mouse Anti Human Pmlc2 Ser19, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti+human+pmlc2+ser19/pmc12304211-309-65-69?v=Cell+Signaling+Technology+Inc
    Average 86 stars, based on 1 article reviews
    mouse anti human pmlc2 ser19 - by Bioz Stars, 2026-08
    86/100 stars
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    a – i Physical interventions altering lumen and branch expansions by externally loading intraluminal pressure in on-chip angiogenesis of ECs. a A schematic diagram showing the timeline of the loading and release cycles of intraluminal pressure. b , c Changes of lumens and branches when loaded followed by release of the additional intraluminal pressure. b Representative DIC images. White dotted lines indicate the proximal and distal boundaries of the analyzed luminal region. c Quantification of the changes in luminal area (loading: n = 12; release: n = 11, from 3 independent experiments). d – i Time-lapse analyses of on-chip angiogenesis during the intervention. d Representative serial DIC images at the elapsed time indicated at the top before and after loading and the release of additional intraluminal pressure (top, See also Supplementary Movie ) and kymograph of branch elongation (bottom). In the DIC images, yellow arrows and red lines indicate the trajectory of the tip of the branch and the tip EC displacement, respectively. In the kymograph, red and blue dotted lines indicate time points at which additional intraluminal pressure was loaded and then released, respectively. e Box plots of averaged branch elongation and the individual plots 3 h before (pressure change (−)) and after (load) the 1 st pressure loading and 3 h after its release (release) (left), and the corresponding box and individual plots in the control group without interventions (right) (pressure loading and release: n = 21 from 4 independent experiments, control: n = 19, from 3 independent experiments, See also Supplementary Fig. ). f – i Kymograph showing the dynamics of tip EC movement for 50 min before and 100 min after loading ( f ) and releasing ( h ) additional intraluminal pressure (time=0, dotted red line), and the quantification showing in box plots of averaged tip EC movement speed ( g , each group: n = 13 from 4 independent experiments; i , each group: n = 8 from 4 independent experiments). j – m Pharmacological interventions altering lumen and branch expansions of on-chip angiogenesis of ECs by treatment with Y27632 (top) and Blebbistatin (bottom), and without and with TG treatment of the gel. j Representative fluorescent images of the angiogenic branches. k Representative confocal z-projection images, with magnified images of square areas bound by dots (z-projection) and their corresponding confocal x-y slice images at specific z-portions (slice), showing localization pattern of pMLC2 in the angiogenic branches around the area where the de novo vascular lumen (yellow asterisks) develops. l , m , Box plots of branch length (left) and Tip expandability (right), without and with Y27632 ( l ) or Blebbistatin ( m ) and without and with TG treatment of the gel ( l , Y27632 (−) TG (−): n = 117; Y27632 ( + ) TG (−): n = 108; Y27632 ( + ) TG (+): n = 102, from 3 independent experiments, ( m ), Blebbistatin (−) TG (−): n = 39; Blebbistatin (+) TG (−): n = 35; Blebbistatin (+) TG (+): n = 64, from 3 independent experiments). All box plots show the interquartile range, with the middle line defining the median, and whiskers show the minimum and maximum values, excluding outliers ( g , i , l , m ). Outlier values are defined as being 1.5 times the interquartile range above and below the third and first quartile, respectively. Scale bars: 20 µm ( d , k ), 25 µm ( b ) and 100 µm ( j ). Two-sided Wilcoxon signed rank test ( c ), Wilcoxon signed rank test with a Bonferroni correction ( e ) and two-sided Mann-Whitney U test with a Bonferroni correction ( g , i , l , m ). Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Biomechanical control of vascular morphogenesis by the surrounding stiffness

    doi: 10.1038/s41467-025-61804-z

    Figure Lengend Snippet: a – i Physical interventions altering lumen and branch expansions by externally loading intraluminal pressure in on-chip angiogenesis of ECs. a A schematic diagram showing the timeline of the loading and release cycles of intraluminal pressure. b , c Changes of lumens and branches when loaded followed by release of the additional intraluminal pressure. b Representative DIC images. White dotted lines indicate the proximal and distal boundaries of the analyzed luminal region. c Quantification of the changes in luminal area (loading: n = 12; release: n = 11, from 3 independent experiments). d – i Time-lapse analyses of on-chip angiogenesis during the intervention. d Representative serial DIC images at the elapsed time indicated at the top before and after loading and the release of additional intraluminal pressure (top, See also Supplementary Movie ) and kymograph of branch elongation (bottom). In the DIC images, yellow arrows and red lines indicate the trajectory of the tip of the branch and the tip EC displacement, respectively. In the kymograph, red and blue dotted lines indicate time points at which additional intraluminal pressure was loaded and then released, respectively. e Box plots of averaged branch elongation and the individual plots 3 h before (pressure change (−)) and after (load) the 1 st pressure loading and 3 h after its release (release) (left), and the corresponding box and individual plots in the control group without interventions (right) (pressure loading and release: n = 21 from 4 independent experiments, control: n = 19, from 3 independent experiments, See also Supplementary Fig. ). f – i Kymograph showing the dynamics of tip EC movement for 50 min before and 100 min after loading ( f ) and releasing ( h ) additional intraluminal pressure (time=0, dotted red line), and the quantification showing in box plots of averaged tip EC movement speed ( g , each group: n = 13 from 4 independent experiments; i , each group: n = 8 from 4 independent experiments). j – m Pharmacological interventions altering lumen and branch expansions of on-chip angiogenesis of ECs by treatment with Y27632 (top) and Blebbistatin (bottom), and without and with TG treatment of the gel. j Representative fluorescent images of the angiogenic branches. k Representative confocal z-projection images, with magnified images of square areas bound by dots (z-projection) and their corresponding confocal x-y slice images at specific z-portions (slice), showing localization pattern of pMLC2 in the angiogenic branches around the area where the de novo vascular lumen (yellow asterisks) develops. l , m , Box plots of branch length (left) and Tip expandability (right), without and with Y27632 ( l ) or Blebbistatin ( m ) and without and with TG treatment of the gel ( l , Y27632 (−) TG (−): n = 117; Y27632 ( + ) TG (−): n = 108; Y27632 ( + ) TG (+): n = 102, from 3 independent experiments, ( m ), Blebbistatin (−) TG (−): n = 39; Blebbistatin (+) TG (−): n = 35; Blebbistatin (+) TG (+): n = 64, from 3 independent experiments). All box plots show the interquartile range, with the middle line defining the median, and whiskers show the minimum and maximum values, excluding outliers ( g , i , l , m ). Outlier values are defined as being 1.5 times the interquartile range above and below the third and first quartile, respectively. Scale bars: 20 µm ( d , k ), 25 µm ( b ) and 100 µm ( j ). Two-sided Wilcoxon signed rank test ( c ), Wilcoxon signed rank test with a Bonferroni correction ( e ) and two-sided Mann-Whitney U test with a Bonferroni correction ( g , i , l , m ). Source data are provided as a Source Data file.

    Article Snippet: The antibodies used were as follows: mouse anti-human CD31 (WM59, BioLegend, 303102), rabbit anti-human PDGFRβ (Y92, OriGene), rabbit anti-GOLPH4 (Abcam, ab28049), rabbit anti-VE-cadherin (D87F2, Cell Signaling, 2500), goat anti-collagen type IV (SouthernBiotech, 1340-01), rabbit anti-laminin (Abcam, ab11575), rabbit anti-NG2 (Millipore, AB5320), rat anti-mouse CD31 (MEC13.3, BD Pharmingen, 552074), rat anti-ICAM2 (3C4, BioLegend, 105601), goat anti-human ARPC2 (Novus Biologicals, NB100-137), rabbit anti-human TRIP10 (CIP4, Proteintech, 10798-1-AP), mouse anti-human pMLC2 (Ser19) (Cell Signaling, #3675), rabbit anti-human Moesin (EPR3864, Abcam, ab52490), Alexa Fluor 488-conjugated rabbit anti-ERG2 (EPR3864, Abcam, ab196374), Alexa Fluor 488-conjugated goat anti-mouse IgG and goat anti-rat IgG (Thermo Fischer Scientific), Cy3-conjugated goat anti-rabbit IgG and goat anti-rat IgG (Thermo Fischer Scientific) and Cy3-conjugated donkey anti-rabbit IgG (Thermo Fischer Scientific).

    Techniques: Control, MANN-WHITNEY